Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs

• Authors: USTIANENKO Dmytro; HROŠŠOVÁ Dominika; POTĚŠIL David; CHALUPNÍKOVÁ Kateřina; HRAZDILOVÁ Kristýna; PACHERNÍK Jiří; CETKOVSKÁ Kateřina; ULDRIJAN Stjepan; ZDRÁHAL Zbyněk; VAŇÁČOVÁ Štěpánka
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: RNA
• MU Faculty or unit: Central European Institute of Technology
• Web: http://rnajournal.cshlp.org/content/early/2013/10/18/rna.040055.113.abstract
• Doi: http://dx.doi.org/10.1261/rna.040055.113
• Field: Genetics and molecular biology
• Keywords: DIS3L2; RNA degradation; RNA uridylation; let-7 miRNA

Attached files

• Ustianenko_et_al_RNA2013.pdf
• ZVV_2013_006_1126856_Mammalian.pdf

• Description: The mechanisms of gene expression regulation by miRNAs have been extensively studied. However, the regulation of miRNA function and decay has long remained enigmatic. Only recently, 3'uridylation via LIN28A-TUT4/7 has been recognized as an essential component controlling the biogenesis of let-7 miRNAs in stem cells. Although uridylation has been generally implicated in miRNA degradation, the nuclease responsible has remained unknown. Here, we identify the Perlman syndromeassociated protein DIS3L2 as an oligo(U)-binding and processing exoribonuclease that specifically targets uridylated pre-let-7 in vivo. This study establishes DIS3L2 as the missing component of the LIN28-TUT4/7-DIS3L2 pathway required for the repression of let-7 in pluripotent cells.

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