Kinetic characterization of cytochrome P450 2C9 hydroxylation of diclofenac by capillary electrophoresis – mass spectrometry

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Authors

LANGMAJEROVÁ Monika ŘEMÍNEK Roman GLATZ Zdeněk

Year of publication 2013
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Web http://www.chemicke-listy.cz/common/content-issue_s3-volume_107-year_2013.html
Field Biochemistry
Keywords capillary electrophoresis; mass spectrometry; on-line connection; enzyme reaction
Attached files
Description Subject of this contribution is application of capillary electrophoresis – mass spectrometry for kinetic characterization of cytochrome P450 2C9 hydroxylation of diclofenac. In this study enzymatic reaction was took place inside the capillary, resulting compounds were detected by MS. Mixing of reaction components in capillary was based on the general principle of diffusion.
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