Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform

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Authors

SCHEJBAL Jan ŘEMÍNEK Roman ZEMAN Lukáš ZEISBERGEROVÁ Marta GLATZ Zdeněk

Year of publication 2013
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Web http://www.chemicke-listy.cz/docs/full/2013_s3_s420-s453.pdf
Field Biochemistry
Keywords Capillary Electrophoresis; Drug Metabolism; CYP2C9; Diclofenac; Immobilization; IMER
Description Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Enzyme immobilization represents a useful approach in this field because of CYP recycling and simplification of sequential analyses by providing protein-free samples. A new method for immobilization of therapeutically important cytochrome P450 2C9 isoform (CYP2C9) on magnetic microparticles SiMAG Carboxyl is thus presented.
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