Defining the selectivity of processes along the auxin response chain: a study using auxin analogues

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Authors

SIMON Sibu KUBEŠ Martin BASTER Pawel ROBERT Stéphanie DOBREV Petre Ivanov FRIML Jiří PETRÁŠEK Jan ZAŽÍMALOVÁ Eva

Year of publication 2013
Type Article in Periodical
Magazine / Source New Phytologist
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1111/nph.12437/abstract;jsessionid=EAA0C3D7357390C89E290911E83AEF3F.f03t02
Doi http://dx.doi.org/10.1111/nph.12437
Field Genetics and molecular biology
Keywords auxin analogues; auxin signalling; auxin transport; indole 3 acetic acid (IAA); indole 3 butyric acid (IBA); naphthalene 1 acetic acid (NAA); 2.4 dichlorophenoxyacetic acid (2.4 D)
Description The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.
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