Metody izolace vybraných proteinů hlíz brambor

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Title in English	Methods for isolating proteins of potato tubers
Authors	BÁRTA Jan BÁRTOVÁ Veronika ZDRÁHAL Zbyněk BRABCOVÁ Adéla KAMENOVÁ Andrea DIVIŠ Jiří
Year of publication	2013
MU Faculty or unit	Central European Institute of Technology
Description	The basic element of the separation process is a two step procedure based on ion exchange and affinity chromatography. For subsequent fractionation a column with ceramic media and gradient elution via FPLC is used. The aim of the methodology is the determination of two fundamental biological activities of proteins tubers - lipidacylhydrolase patatin activity and activities of protease inhibitors.
Related projects:	Evaluation of wild potato tuber proteins potential for utilization in potato (Solanum tuberosum L.) breeding and biotechnological applications