Comparison of two electrophoretic methods with minimum consumption of protein sample for determination of the binding constant

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Authors

MICHALCOVÁ Lenka GLATZ Zdeněk

Year of publication 2014
Type Article in Proceedings
Conference Acta Universitatis Palackianae Olomucensis Chemica 51S
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords binding constant, capillary electrophoresis, HD method, CE-FA
Description The binding constant of model system (BSA - salicylic acid) was measured by capillary electrophoresis – frontal analysis and Hummel Dreyer methods with external and internal calibrations. Their results were compared according to simplicity, repeatability, calibration procedure and scatter of data points.
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