Study of competitive binding between diclofenac and tryptophan on hunam serum albumin using capillary electrophoresis
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014. |
| MU Faculty or unit | |
| Citation | |
| Web | Oficiální stránky konference |
| Field | Biochemistry |
| Keywords | capillary electrophoresis - frontal analysis; competitive binding; HSA; diklofenak |
| Description | The capillary electrophoresis fontal analysis (CE-FA) was used to study the affinities between diclofenac and tryptophan, and human serum albumin (HSA). Apparent log Kb = (4.4 ± 0.01) L/mol for diclofenac–HSA and log Kb = (3.95 ± 0.004) L/mol for tryptophan–HSA have a good repeatability. The displacement experiment shows the displacement of tryptophan from binding site using diclofenac. |
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