Polymorphism 140A/G in lactotransferrin gene and its association with development of dental caries and gingivitis in children
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Introduction: Dental caries and periodontal diseases are complex, multifactorial diseases and one of the most common illnesses worldwide. Recent evidence suggests a role of LTF in caries and periodontitis. The aim of this study was determine the frequency of the polymorphism 140A/G in lactotransferrin gene and its association with dental caries and gingivitis in children in Czech population. Material and Methods: School children aged 11 – 13 years were included in the study (N = 637). The subjects were divided into two groups: without caries (DMFT = 0) and with caries (DMFT1). The caries affected group was stratified as low (DMFT=1), moderate (2DMFT3) and high (DMFT4) caries experience. The level of gingivitis was rated according to gingival index (GI) and subjects were divided into the groups without (GI = 0) and with (GI1) gingivitis. The LTF rs1126478 (140A/G exon 2, Lys/Arg) genotypes were determined by polymerase-chain reaction (PCR) with restriction analysis by EarI enzyme. Results: Of 637 subjects, 155 (24.3%) were caries free children. There were no statistical significant differences between caries levels and allele or genotype distributions in the total cohort. When the caries affected group (N=482) was stratified as low (DMFT=1), moderate (2DMFT3) and high (DMFT4) caries experience, allele and genotype frequencies were similar among all subgroups. Gingivitis was diagnosed in 53.4% children. The difference in frequencies of alleles and genotypes between the group of healthy children and children with gingivitis was also not significant (p0.05). Conclusion: The polymorphism 140A/G in lactotransferrin gene was not associated with the susceptibility and severity of dental caries and gingivitis in the Czech population. |
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