Characterization of Human Uropathogenic ESBL-Producing Escherichia coli in the Czech Republic: Spread of CTX-M-27-Producing Strains in a University Hospital

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Authors

MICENKOVÁ Lenka ŠIŠKOVÁ Petra BOSÁK Juraj JAMBOROVÁ Ivana ČERNOHORSKÁ Lenka ŠMAJS David

Year of publication 2014
Type Article in Periodical
Magazine / Source Microbial Drug Resistance
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1089/mdr.2014.0013
Field Microbiology, virology
Keywords SPECTRUM BETA-LACTAMASES; CTX-M-TYPE; FIELD GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; URINARY-TRACT-INFECTIONS; MOLECULAR EPIDEMIOLOGY; VIRULENCE DETERMINANTS; ANTIBIOTIC-RESISTANCE; KLEBSIELLA-PNEUMONIAE; PHYLOGENETIC GROUP
Description Aims: The purpose of this study was to characterize the extended-spectrum b-lactamase (ESBL)-producing uropathogenic Escherichia coli (UPEC) strains isolated in the South Moravia region of the Czech Republic. Results: Out of 109 ESBL-producing UPEC isolates, the CTX-M-15-producing E. coli O25b-ST131 was detected in 55 (50.5%) and the CTX-M-27-producing E. coli O25b-ST131 in 40 isolates (36.7%). Most isolates were distributed among three pulsed-field gel electrophoresis clusters and were characterized by low variability relative to antibiotic resistance patterns, in E. coli phylogroups and by the prevalence of virulence and bacteriocin determinants. Despite this, 14 groups of identical isolates (comprising a total of 41 isolates) were identified when all tested parameters of E. coli were combined. Conclusions: Since the occurrence of E. coli B2-O25b-ST131 CTX-M-27 was only recently described in Asia, the frequent isolation of this lineage among patients in South Moravia suggests an efficient transfer of this clone from Asian countries. The limited variability of detected parameters of ESBL-producing UPEC strains is consistent with a common origin of the analyzed isolates, in which there is an ongoing process of genetic diversification.
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