Kinetic Study OF Ketamine N-Demethylation Mediated by On-Line Enantioselective Capillary Electrophoresis Method

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Authors

ŘEMÍNEK Roman GLATZ Zdeněk THORMANN Wolfgang

Year of publication 2014
Type Article in Proceedings
Conference 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Kapilární elektroforéza; Enantioselektivní separace; Cytochrom P450; CYP3A4; Ketamin
Description Enantioselective discrimination is an important phenomenon influencing pharmacokinetics and pharmacodynamics of chiral drugs in humans and animals. Metabolism appraisal of single enantiomers of every drug candidate compound thus constitutes the inseparable part of LADME/tox screening. Capillary electrophoresis represents a promising technique in the field of in vitro enzyme assays due to miniscule sample consumption, simple implementation of chiral separations and high-throughput by automation. An optimized and economical capillary electrophoretic method for on-line studies of the enantioselective drug metabolism mediated by cytochrome P450 3A4 enzyme (CYP3A4) is introduced.
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