p- SRM, SWATH a HRM – cílené proteomické přístupy na hmotnostním spektrometru TripleTOF 5600+ a jejich aplikace v onkologickém výzkumu

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Title in English p- SRM, SWATH and HRM – Targeted Proteomics Approaches on TripleTOF 5600+ Mass Spectrometer and Their Applications in Oncology Research
Authors

FAKTOR Jakub MICHALOVÁ Eva BOUCHAL Pavel

Year of publication 2014
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords proteomics; selected reaction monitoring; oncology; SWATH; biomarkers; molecula diagnostics
Description Development of novel diagnostic and therapeutic approaches in cancer research requires sensitive and quantitative assays for determination of cancer-associated proteins in clinical samples. Novel quantitative targeted proteomic approaches are overviewed in this communication. A major advantage of selected reaction monitoring (SRM) and pseudo- SRM lies in the selective and sensitive quantifi cation of selected proteins in large sample sets. As such, they represent an alternative to immunochemical approaches. On the other hand, the potential of HRM and SWATH lies in recording of digital fi ngerprints, which enable post-acquisition quantitative proteomic data mining on a similar basis to SRM. This article shows applications of targeted proteomics in a number of cancer research studies where they were used for quantifi cation and validation of current or potential protein bio markers and to study their role in cancer development and progression.
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