Analýza malých změn Fc-glykosylace IgG s využitím izotopového značení glykopeptidů

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Title in English Analysis of IgG Fc-glycosylation using glycopeptide stable isotope labeling
Authors

PABST Martin BENEŠOVÁ Iva FAGERER Stephan Rupert JACOBSEN Mathias EYER Klaus SCHMIDT G. KRISMER Jasmin WAHL F. PREISLER Jan ZENOBI Renato

Year of publication 2015
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description For the first time, stable isotope labeling of the glycopeptide was used for IgG N-glycosylation analysis. Succinic anhydride was found as a suitable label as it can be prepared in natural (light) and heavy (D413C4) form. A sample preparation protocol consisting of an in-solution tryptic digestion, a „one-pot“ labeling and a SPE was developed. Labeled glycopeptides were analyzed using MALDI MS and LC-ESI MS.
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