Label-free bead-based metallothionein electrochemical immunosensor

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Authors

NEJDL Lukas NGUYEN Hoai Viet RICHTERA Lukáš KŘÍŽKOVÁ Soňa GURÁŇ Roman MASAŘÍK Michal HYNEK David HEGER Zbynek LUNDBERG Karin ERIKSON Kristofer ADAM Vojtěch KIZEK René

Year of publication 2015
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Medicine

Citation
Web http://dx.doi.org/10.1002/elps.201500069
Doi http://dx.doi.org/10.1002/elps.201500069
Field Biochemistry
Keywords Immunosensor; Label-free; Metallothionein; Superparamagnetic particles
Description A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 mikroL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 mikrog/mL) in real samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.
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