Diagnostic panel for foodborne pathogens

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

RESLOVÁ Nikol KAŠNÝ Martin SLANÝ Michal KOUDELA Břetislav KRÁLÍK Petr

Year of publication 2015
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
Description The changes in farming practices towards bio-production, globalization of food market, global climate change and also the way of food processing facilitate the risks of widespread disseminations of foodborne diseases, including the pathogens of protozoa and multicellular parasites such as Toxoplasma gondii, Trichinella spiralis or Taenia saginata. These facts consequently induce the need of improvement of the diagnostic tools for foodborne agents in the final food products such as meat. In relation to this problematics, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, representing the novel diagnostic platform based on magnetic microspheres and visualization realized via MAGPIX instrument for qualitative and quantitative readout of signal. This modern approach enables simultaneous direct screen of complex samples potentially containing the DNA originating from number of different parasitic organisms. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. The calibration of the system and optimization of method is in process.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.