Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization

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Authors

CELÁ Andrea MÁDR Aleš DĚDOVÁ Tereza PELCOVÁ Marta GLATZ Zdeněk

Year of publication 2015
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Combination of capillary electrophoresis with laser-induced fluorescence detection provides high separation efficiency and sensitivity of the detection. Amino acids, which are very important for living organisms, and their levels in biological fluids can reveal health state of organisms, exhibit very low or no native fluorescence. As a result time consuming and laborious derivatization procedure has to be performed. Inner space of narrow capillary used in capillary electrophoresis can be used both for separation and derivatization. Optimization and comparison of pre-capillary and on-capillary derivatization of amino acids are subjects of this contribution.
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