Exacerbation of Substrate Toxicity by IPTG in Escherichia coli BL21(DE3) Carrying a Synthetic Metabolic Pathway

Investor logo
Investor logo

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

DVOŘÁK Pavel CHRÁST Lukáš NIKEL Pablo Ivan FEDR Radek SOUČEK Karel SEDLÁČKOVÁ Miroslava CHALOUPKOVÁ Radka LORENZO DE Víctor PROKOP Zbyněk DAMBORSKÝ Jiří

Year of publication 2015
Type Article in Periodical
Magazine / Source Microbial Cell Factories
MU Faculty or unit

Faculty of Science

Citation
Web http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/12/Dvorak_2015MCF.pdf
Doi http://dx.doi.org/10.1186/s12934-015-0393-3
Field Microbiology, virology
Keywords Metabolic burden; Substrate toxicity; Escherichia coli; Heterologous metabolic pathway; Isopropyl beta-D-1-thiogalactopyranoside; Lactose; 1.2.3-trichloropropane; Metabolic engineering
Description Background: Heterologous expression systems based on promoters inducible with isopropyl-beta-D-1- thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate LacIQ/PlacUV5-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of the exogenous genes during a bioprocess. This burden originates from a combination of stresses that may include competition for the expression machinery, sidereactions due to the activity of the recombinant proteins, or the toxicity of their substrates, products and intermediates. However, the physiological impact of IPTG-induced conditional expression on the recombinant host under such harsh conditions is often overlooked. Results: The physiological responses to IPTG of the E. coli BL21(DE3) strain and three different recombinants carrying a synthetic metabolic pathway for biodegradation of the toxic anthropogenic pollutant 1,2,3-trichloropropane (TCP) were investigated using plating, flow cytometry, and electron microscopy. Collected data revealed unexpected negative synergistic effect of inducer of the expression system and toxic substrate resulting in pronounced physiological stress. Replacing IPTG with the natural sugar effector lactose greatly reduced such stress, demonstrating that the effect was due to the original inducer’s chemical properties. Conclusions: IPTG is not an innocuous inducer; instead, it exacerbates the toxicity of haloalkane substrate and causes appreciable damage to the E. coli BL21(DE3) host, which is already bearing a metabolic burden due to its content of plasmids carrying the genes of the synthetic metabolic pathway. The concentration of IPTG can be effectively tuned to mitigate this negative effect.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.