Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus
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| Year of publication | 2015 |
| Type | Conference abstract |
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| Description | Introduction: Vitamin D possesses a plethora of extraskeletal functions including regulation of cell proliferation, maturation and apoptosis, angiogenesis and is also affects insulin secretion and possibly insulin action. Via binding to vitamin D receptor (VDR) it regulates expression of hundreds of genes. A possible role of vitamin D in gestational diabetes (GDM) pathogenesis has been proposed. Several studies showed that vitamin D levels are lower in women with GDM compared to those with normal pregnancy. In our recent study we found that mid-gestational vitamin D levels did not significantly differ between pregnant women with GDM and healthy controls, while postpartum vitamin D levels raised significantly in both groups and in women with GDM history remained significantly lower compared to controls (Pleskáčová et al., 2015). Little is known about prevalence of VDR single nucleotide polymorphisms (SNP) and their possible relationship to plasma vitamin D levels in pregnant women with and without GDM. The aims of this study are to (i) compare allele and genotype frequencies of VDR SNPs between pregnant women with and without GDM and (ii) to analyze a relationship between VDR SNPs and vitamin D levels. Methods: Study comprised a total of 229 pregnant women with (n = 149) and without (n = 80) GDM. Genotyping for VDR single nucleotide polymorphisms (rs731236, rs7975232, rs1544410, rs2228570) was performed by real time PCR using TaqMan® assays. In a subgroup of women (47 with and 29 without GDM) we had two samples of vitamin D level: first, 24–30th week of gestation and second, 6 weeks–12 months after delivery. Results: Comparison of allele and genotype frequencies of studied SNPs did not show any significant difference between women with GDM and control group (P > 0.05, chi-square and Fisher-exact test). However, we found correlation between VDR SNP rs7975232 and vitamin D level postpartum in women with GDM (P = 0.0096, Kruskal-Wallis ANOVA). The AA genotype was associated with lower 25(OH)D level compared to other genotypes (P = 0.0066, Kruskal-Wallis ANOVA). Conclusion: In our previous study we confirmed overall high prevalence of vitamin D deficiency in pregnant women, without significant differences between GDM and control group. Although vitamin D levels in both groups were higher postpartum than during pregnancy, in women with previous GDM remained significantly lower than in control group – this was associated with AA genotype in VDR polymorphism (rs7975232). |
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