Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

• Authors: MLYNÁRIKOVÁ Katarína; SAMEK Ota; BERNATOVÁ Silvie; RŮŽIČKA Filip; JEŽEK Jan; HÁRONIKOVÁ Andrea; ŠILER Martin; ZEMÁNEK Pavel; HOLÁ Veronika
• Year of publication: 2015
• Type: Article in Periodical
• Magazine / Source: Sensors
• MU Faculty or unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.3390/s151129635
• Field: Microbiology, virology
• Keywords: Raman spectroscopy; bacteria; yeasts; culture media
• Description: Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.

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