Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications

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Authors

ABDEL-RAHMAN Rasha M. ABDEL-MOHSEN A. M. HRDINA Radim BURGERT Ladislav FOHLEROVÁ Zdenka PAVLIŇÁK David SAYED O.N. JANČÁŘ Josef

Year of publication 2016
Type Article in Periodical
Magazine / Source International Journal of Biological Macromolecules
MU Faculty or unit

Faculty of Science

Citation
Web odkaz na článek
Doi http://dx.doi.org/10.1016/j.ijbiomac.2016.04.087
Field Plasma physics
Keywords Hyaluronan;chitosan;nonwoven fabrics;cytotoxicity;healing properties
Description Thin layers of chitosan (positively charged)/ sodium hyaluronate (negatively charged)/ nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/ nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different tools such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E.coli) and Streptococcus aureus (St. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics 4 interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
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