Způsob absolutní kvantifikace exprese miRNA, zejména miR-34a a/nebo miR-150, a jeho použití v diagnostice a prognostice B-buněčných malignit

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Title in English Absolute quantification method of miRNA expression, particularly that of miR-34a and/or miR-150 and their use in diagnostics and prognostics of B-cell malignancies
Authors

MRÁZ Marek ČERNÁ Kateřina POSPÍŠILOVÁ Šárka MAYER Jiří

Year of publication 2016
Type Patent
MU Faculty or unit

Central European Institute of Technology

Publisher Úřad průmyslového vlastnictví
State Czech Republic
Patent's number 306080
Web https://isdv.upv.cz/webapp/webapp.pts.det?xprim=10098590&lan=cs&s_majs=&s_puvo=&s_naze=&s_anot=
Description The present invention provides an absolute quantification method of miRNA expression, being preferably selected from miR-34a and/or miR-150, wherein miRNA expression, being preferably selected from miR-34a and/or miR-150, along with the expression of endogenous check, which is a small nucleic RNA or permanently expressed miRNA are determined in a biological sample being withdrawn from a patient body. At the same time, there is determined expression of synthetic standards of miRNA, being preferably selected from miR-34a and/or miR-150 and endogenous check of known number of molecules. The invention further provides the use of the above-indicated quantification method for in vitro diagnostics of B-cell malignancies, prognostics of B-cell malignancies and prediction of therapeutic response of patients with B-cell malignancies, especially CLL, identification of TP53 abnormalities.
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