Validation of Minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing Klebsiella pneumoniae isolates in tertiary care hospital
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Periodical |
| Magazine / Source | Diagnostic Microbiology and Infectious Disease |
| MU Faculty or unit | |
| Citation | |
| Web | http://ac.els-cdn.com/S0732889316300505/1-s2.0-S0732889316300505-main.pdf?_tid=1e0a88a6-84be-11e6-a6d2-00000aab0f6c&acdnat=1474986456_687cb42e2b0877c46e8213dd759647c5 |
| Doi | http://dx.doi.org/10.1016/j.diagmicrobio.2016.03.010 |
| Field | Microbiology, virology |
| Keywords | Klebsiella pneumoniae; ESBL; High-resolution melt analysis; Multi-locus sequence typing; Minim typing |
| Attached files | |
| Description | Minim typing is derived from the multi-locus sequence typing (MIST). It targets the same genes, but sequencing is replaced by high resolution melt analysis. Typing can be performed by analysing six loci (6MelT), four loci (4MelT) or using data from four loci plus sequencing the tonB gene (HybridMelT). The aim of this study was to evaluate Minim typing to discriminate extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KLPN) isolates at our hospital. In total, 380 isolates were analyzed. The obtained alleles were assigned according to both the 6MelT and 4MelT typing scheme. In 97 isolates, the tonB gene was sequenced to enable HybridMelT typing. We found that the presented method is suitable to quickly monitor isolates of ESBL-KLPN; results are obtained in less than 2 hours and at a lower cost than MLST. We identified a local ESBL-KLPN outbreak and a comparison of colonizing and invasive isolates revealed a long term colonization of patients with the same strain. |
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