Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.

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Authors

ŠKORPÍKOVÁ Lucie KAŠNÝ Martin SLANÝ Michal KRÁLÍK Petr GELNAR Milan ILGOVÁ Jana

Year of publication 2016
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
Description Nematodes of the genus Trichinella are clinically important parasites invading cells of the small intestine and subsequently skeletal muscles. Their global distribution is corresponding to the wide range of their hosts involving birds, reptiles and mammals. Seven (T1, T2, T3, T4, T5, T6 a T10) of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious and sometimes fatal human disease. During the life cycle, the individuals of this nematode parasite release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. These molecules are essential for many biological processes like development, digestion, migration, and also in manipulation of the host immunne system. The most dominant components of the E-S products are cystatins – inhibitors of cysteine peptidases of C1 and rarely of C13 family. Our work is focused on the characterization of „multi cystatin-like domain“ (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared. The recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnosis of parasitic nematodes from the genus Trichinella. We employed the xMAP technology - novel diagnostic platform combining immunoassay approach and magnetic microspheres.
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