Capillary Electrophoresis-Frontal Analysis For The Characterization Of Drug-Metal Binding

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Authors	MICHALCOVÁ Lenka ASMARI Mufarreh GLATZ Zdeněk EL DEEB Sami
Year of publication	2016
Type	Article in Proceedings
Conference	13 th International Interdisciplinary Meeting on Bioanalysis CECE 2016
MU Faculty or unit	Faculty of Science
Citation
Web	Oficiální stránky konference
Field	Biochemistry
Keywords	capillary electrophoresis - frontal analysis; drug - metal binding
Description	This study examined the possibilities of using capillary electrophoresis-frontal analysis (CE-FA) for the characterization of drug-metal binding parameters such as formation constant (Kf) and number of binding sites. Deferiprone and iron in the Fe(III) form were selected as model system. Apparent Kf values were obtained using nonlinear regression. The results provide a more quantitative picture of how this drug binds with metal and illustrate how CE FA can be used to examine drug metal interactions.
Related projects:	Centrum pokročilých bioanalytických technologií Podpora biochemického výzkumu v roce 2016 Podpora biochemického výzkumu v roce 2017