Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Proceedings |
| Conference | CECE 2016 13th International Interdisciplinary meeting on bioanalysis |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | MALDI MS; ESI MS; SALD ICP MS; metallothioneins |
| Description | We present a new methodology for coupling conventional on-line LC-ESI MS with two off-line detection techniques: matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) analyses. The Off-line approach is facilitated using a commercial MALDI target made of conductive plastics, which is suitable for the both MALDI MS and SALD ICP MS. Due to this arrangement, two types of analysis, molecular and elemental determination of metallothionein 1 (MT-1) were performed. |
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