Changes in equol and major soybean isoflavone contents during processing and storage of yogurts made from control or isoflavone-enriched bovine milk determined using LC-MS (TOF) analysis

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Authors

KAŠPAROVSKÁ Jitka DADÁKOVÁ Kateřina LOCHMAN Jan HADROVÁ Sylvie KŘÍŽOVÁ Ludmila KAŠPAROVSKÝ Tomáš

Type Article in Periodical
Magazine / Source Food Chemistry
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.foodchem.2016.12.010
Field Biochemistry
Keywords Phytoestrogens; Isoflavones; Bovine milk; Equol; Dairy products; Storage
Description The effect of supplementing a basal diet for dairy cows with "Soybean extract 40% (Biomedica, Prague, Czech Republic), containing 40% soybean isoflavones, on the contents of daidzein, glycitein, genistein, and equol in milk as well as fresh and mature yogurts was estimated. To determine the contents of these isoflavonoids, an efficient analytical LC-MS (TOF) technique was used. The "Soybean extract 40" used in our study contained an especially high proportion of daidzein (307 g kg(-1)). In both milk and yogurt samples, the amounts of daidzein and its metabolite equol were significantly higher in samples obtained from cows that received the isoflavone extract-supplemented diet than from those that received the basal diet, as the precursor daidzein contributed to the increased equol concentrations. Fermentation caused significant changes in the daidzein and glycitein concentrations. With maturation, the concentrations of daidzein and equol were unaffected, while the glycitein concentration decreased significantly. (C) 2016 Elsevier Ltd. All rights reserved.
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