PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

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Authors

SIMON Sibu SKUPA Petr VIAENE Tom ZWIEWKA Marta TEJOS Ricardo KLIMA Petr CARNA Maria ROLCIK Jakub DE RYCKE Riet MORENO Ignacio DOBREV Petre I. ORELLANA Ariel ZAZIMALOVA Eva FRIML Jiri

Year of publication 2016
Type Article in Periodical
Magazine / Source New Phytologist
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1111/nph.14019/abstract;jsessionid=10FCD031F6E3F17A23746DD5AD2F3EF2.f04t04
Doi http://dx.doi.org/10.1111/nph.14019
Field Genetics and molecular biology
Keywords auxin; endoplasmic reticulum (ER); lateral root; PIN; plasma membrane (PM)
Description Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
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