Chromosome Preparation for Cytogenetic Analyses in Arabidopsis

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Authors

MANDÁKOVÁ Terezie LYSÁK Martin

Year of publication 2016
Type Article in Periodical
Magazine / Source Current protocols in plant biology
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/cppb.20009/abstract
Doi http://dx.doi.org/10.1002/cppb.20009
Field Genetics and molecular biology
Keywords arabidopsis; Brassicaceae; mitotic and meiotic chromosomes; pachytene
Description Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step-by-step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana. Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae).
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