Characterization of lectin AFL from opportunistic pathogen Aspergillus fumigatus – combining a broad spectrum of biophysical methods

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Authors	HOUSER Josef KOMÁREK Jan HORÁČKOVÁ Aneta WIMMEROVÁ Michaela
Year of publication	2017
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	Detailed protein characterization is a pivotal step for understanding its role in organism, deciphering its functions and its utilization in medicine or industry. The methodology evolves constantly with the progress in biology, physics, mathematics and informational technologies. Nowadays, a broad spectrum of biophysical techniques is available to determine protein purity, homogeneity, folding and stability, to help in structure solution or to examine protein activity and binding properties. Herewith, we demonstrate a case of the lectin AFL from the Aspergillus fumigatus fungus. The combination of various techniques including GPC, DLS, DSF, TSA, DSC, AUC, CD, ITC, SPR, MST and fluorescence microscopy allows for a complete description of the protein behavior. This may bring an essential information to decipher the processes causing A. fumigatus pathogenicity and be a key to the development of potential therapeutics.
Related projects:	Czech Infrastructure for Integrative Structural Biology CEITEC 2020