Cyclopropylamine plasma polymers for increased cell adhesion and growth

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Authors

MANAKHOV Anton LANDOVÁ Marie MEDALOVÁ Jiřina MICHLÍČEK Miroslav POLCAK Josef NEČAS David ZAJÍČKOVÁ Lenka

Year of publication 2017
Type Article in Periodical
Magazine / Source Plasma processes and polymers
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/ppap.201600123/abstract;jsessionid=F73F366060DF262E0A74149729358262.f03t01
Doi http://dx.doi.org/10.1002/ppap.201600123
Field Plasma physics
Keywords amine coatings; cell adhesion; cyclopropylamine; ESCA/XPS; pulsed discharges
Description Plasma polymers with NHx groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NHx decreased with increasing average power P-av, whereas the layer dissolution in water decreased and high P-av led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine-rich layers should enhance the cell proliferation, the WST-1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7-10% of NHx.
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