Sada pro stanovení analytů, její příprava a způsob stanovení analytů

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Title in English A set for determining of analytes, its preparation and the method of determining analytes
Authors

BITTNER Michal JARQUE ORTIZ Sergio HILSCHEROVÁ Klára

Year of publication 2017
Type Patent
MU Faculty or unit

Faculty of Science

Publisher Úřad průmyslového vlastnictví
State Czech Republic
Patent's number 306626
web https://isdv.upv.cz/webapp/webapp.pts.det?xprim=10158318&lan=cs&s_majs=&s_puvo=&s_naze=&s_anot=
Description The invention provides a kit for determination of analyte by a recombinant yeast assay, characterized in that said kit contains a first container with lyophilized nutrient medium, a second container with lyophilized recombinant yeast containing a receptor for the analyte and a reporter gene under transcriptional control of this receptor, and a third container with a frozen reagent for the detection of the expression of the reporter gene; as well as a method of preparation thereof. Further, the invention provides a method for determining the analyte in an aqueous sample using the kit according to any one of claims 1 to 3, charakterized in that it comprises the following steps: - the aqueous sample is transferred to the first container with the lyophilized nutrient medium for the yeast; - the aqueous sample with the dissolved components of the yeast nutrient medium is transferred from the first container to the second container with the lyophilized yeast and incubated; - the reagent for the detection of expression of the reporter gene is transferred from the third container to the second container or the incubated sample is transferred from the second container into the third container, and the expression of the reporter gene is detected.
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