Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

This publication doesn't include Faculty of Arts. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors

DŽATKO Šimon KRAFČÍKOVÁ Michaela HANSEL-HERTSCH R. FESSL T. FIALA Radovan LOJA Tomáš KRAFČÍK Daniel MERGNY J.L. TRANTÍRKOVÁ Silvie TRANTÍREK Lukáš

Type Article in Periodical
Magazine / Source Angewandte Chemie International Edition
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1002/anie.201712284
Keywords DNA; i-motifs; in-cell NMR spectroscopy; structural biology
Description C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.
Related projects: