Evaluation of matrix layer homogeneity utilizing quasi-homogeneous biological tissues in MALDI MSI
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| Year of publication | 2018 |
| Type | Conference abstract |
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| Description | At the present, the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) focuses on increasing several parameters such as the speed of analysis, reproducibility of sample preparation, high spatial resolution and efficient statistical data processing. One of the most critical steps in the MALDI MSI workflow is the application of a homogeneous matrix layer with efficient analyte extraction and reduced spatial diffusion. The balanced compromise between these two aspects of matrix deposition should let to high sensitivity and homogeneous signal distribution. As a result, the chemical map corresponding more with the real distribution in the investigated tissue is yielded. Matrices evince strongly variable crystallization on different tissues. Unfortunately, natural untreated tissue cannot be used to study behavior and characterization of the matrix layer because the tissue itself usually have diverse structures with varying analyte distributions. Therefore, we introduced two biological materials and several treatment processes for the matrix layer characterization independent of the used deposition system, place, and time of preparation have been introduced. |
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