The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Microscopy |
| MU Faculty or unit | |
| Citation | |
| Web | Full Text |
| Doi | http://dx.doi.org/10.1111/jmi.12771 |
| Keywords | biopolymeric scaffold;mesenchymal stem cells;SEM/EDX;tissue engineering; X-ray computed nanotomography |
| Description | This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Asample of highly porous collagen scaffold seeded with contrastedmesenchymalstemcells(MSC)was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. |
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