SEMIQUANTITATIVE ASSESSMENT OF DISHEVELLED-3 PHOSPHORYLATION STATUS BY MASS SPECTROMETRY

Warning

This publication doesn't include Faculty of Arts. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors	HANÁKOVÁ Kateřina POTĚŠIL David BERNATÍK Ondřej ČERVENKA Igor RÁDSETOULAL Matěj BRYJA Vítězslav ZDRÁHAL Zbyněk
Year of publication	2018
Type	Article in Periodical
Magazine / Source	HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1515/hjic-2018-0002
Keywords	phosphorylation; Dishevelled 3; mass spectrometry; CK1 epsilon; NEK2
Description	The focus of this paper is the human Dishevelled 3 protein (hDvl3), an essential component of the Wnt signalling pathway that contributes to their regulation. Mass spectrometry-based analysis of hDvl3 phosphorylations induced by eight associated kinases was performed revealing several dozens of phosphorylation sites. The main outcome of this study was the description of Dvl phosphorylation "patterns" induced by individual kinases.
Related projects:	Dishevelled - identifikace základních koordinát Czech Infrastructure for Integrative Structural Biology CEITEC 2020