MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

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Authors	KOPKOVÁ Alena ŠÁNA Jiří FADRUS Pavel MACHÁČKOVÁ Táňa VEČEŘA Marek VYBÍHAL Václav JURÁČEK Jaroslav VYCHYTILOVÁ Petra SMRČKA Martin SLABÝ Ondřej
Year of publication	2018
Type	Article in Periodical
Magazine / Source	Plos one
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1371/journal.pone.0208580
Keywords	MicroRNA; cerebrospinal fluid
Description	Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.
Related projects:	Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů