Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis

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Authors

CHOCHOLOVÁ Eva DROZDOVÁ Eva FIALOVÁ Dana BRZOBOHATÁ Kristýna CHOCHOLA Václav

Year of publication 2019
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Diagnosis of tuberculosis from historical specimens has been mostly based on detection of IS6110 by PCR and following gel electrophoresis. Even when some authors stressed the possibility that this insertion sequence may have similar counterparts in other microorganisms (e.g. soil bacteria), it continued to be used widely, sometimes without sequencing of the PCR product. This work is aiming at IS6110 and two other sequences that are potential targets for DNA detection of Mycobacteria tuberculosis complex. These three loci are compared by bioinformatic and phylogenetic analysis to assess their usability in ancient DNA studies.
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