Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis
| Authors | |
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| Year of publication | 2019 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Diagnosis of tuberculosis from historical specimens has been mostly based on detection of IS6110 by PCR and following gel electrophoresis. Even when some authors stressed the possibility that this insertion sequence may have similar counterparts in other microorganisms (e.g. soil bacteria), it continued to be used widely, sometimes without sequencing of the PCR product. This work is aiming at IS6110 and two other sequences that are potential targets for DNA detection of Mycobacteria tuberculosis complex. These three loci are compared by bioinformatic and phylogenetic analysis to assess their usability in ancient DNA studies. |
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