Mass spectrometry identification and validation of cell surfaceome associated with breast cancer cell migration and metastasis

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Authors

FAKTOR Jakub KNOPFOVÁ Lucia PÁRALOVÁ Vendula BOUCHALOVÁ Pavla BENEŠ Petr BOUCHAL Pavel

Year of publication 2019
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, we selected and characterized a more migrating subpopulation of MDA-MB-231 breast cancer cells. We applied quantitative mass spectrometry with SILAC labeling to analyze their biotinylated surfaceome and compared it with that of parental MDA-MB-231 cells. Among 838 identified proteins (FDR < 0.01), 210 differentially abundant cell surface proteins with at least one transmembrane domain were found. Of these, (i) catechol-O-methyltransferase (COMT) was successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer and tumor grade by targeted data extraction from the previously acquired SWATH-MS data set of 96 breast cancer tissues [1], and (ii) desmocollin-1 (DSC1) was verified as a protein connected with lymph node status of luminal A breast cancer, tumor grade and Her-2 status by immunohistochemistry in the same set of patients. Kaplan-Meier analysis associated COMT and DSC1 with decreased overall survival in breast cancer subsets. Our findings indicate importance of both proteins for breast cancer progression and metastasis.
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