Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

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Authors

SOCHOR Jiri RYVOLOVA Marketa KRYSTOFOVA Olga SALAS Petr HUBALEK Jaromir ADAM Vojtech TRNKOVÁ Libuše HAVEL Ladislav BEKLOVÁ Miroslava ZEHNALEK Josef PROVAZNIK Ivo KIZEK René

Year of publication 2010
Type Article in Periodical
Magazine / Source Blood Cells, Molecules and Diseases
MU Faculty or unit

Faculty of Science

Citation
Web https://www.mdpi.com/1420-3049/15/12/8618
Doi http://dx.doi.org/10.3390/molecules15128618
Keywords antioxidant activity; spectrometry; trolox; gallic acid
Description The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO(5). Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox (R) and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data provided good correlations between studied methods (R = 0.97 - 0.99).
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