Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor
| Authors | |
|---|---|
| Year of publication | 2020 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Separation Science |
| MU Faculty or unit | |
| Citation | |
| Web | https://doi.org/10.1002/jssc.202000412 |
| Doi | http://dx.doi.org/10.1002/jssc.202000412 |
| Keywords | Enzymes; Assay; Capillary electrophoresis; IMER |
| Description | CE is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogenous or heterogenous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with CE. A number of magnetic nano/microparticle types were tested for this purpose, showing that aldehyde oxidase was most active when immobilized on bare silica magnetic nanoparticles. |
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