Mechanism-Based Design of Efficient PET Hydrolases

Investor logo
Investor logo

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

WEI Ren VON HAUGWIT Gerlis PFAFF Lara MIČAN Jan BADENHORST Christoffel P. S. LIU Weidong WEBER Gert AUSTIN Harry P. BEDNÁŘ David DAMBORSKÝ Jiří BORNSCHEUER Uwe T.

Year of publication 2022
Type Article in Periodical
Magazine / Source ACS Catalysis
MU Faculty or unit

Faculty of Science

Citation
Web https://pubs.acs.org/doi/10.1021/acscatal.1c05856
Doi http://dx.doi.org/10.1021/acscatal.1c05856
Keywords Hydrolase; enzymatic degradation; interfacial biocatalysis; plastic recycling; protein engineering; polyethylene terephthalate (PET); product inhibition; thermostability
Description Polyethylene terephthalate (PET) is the most widespread synthetic polyester, having been utilized in textile fibers and packaging materials for beverages and food, contributing considerably to the global solid waste stream and environmental plastic pollution. While enzymatic PET recycling and upcycling have recently emerged as viable disposal methods for a circular plastic economy, only a handful of benchmark enzymes have been thoroughly described and subjected to protein engineering for improved properties over the last 16 years. By analyzing the specific material properties of PET and the reaction mechanisms in the context of interfacial biocatalysis, this Perspective identifies several limitations in current enzymatic PET degradation approaches. Unbalanced enzyme-substrate interactions, limited thermostability, and low catalytic efficiency at elevated reaction temperatures, and inhibition caused by oligomeric degradation intermediates still hamper industrial applications that require high catalytic efficiency. To overcome these limitations, successful protein engineering studies using innovative experimental and computational approaches have been published extensively in recent years in this thriving research field and are summarized and discussed in detail here. The acquired knowledge and experience will be applied in the near future to address plastic waste contributed by other mass-produced polymer types (e.g., polyamides and polyurethanes) that should also be properly disposed by biotechnological approaches.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.