Koncentrace lysozymu ve slinách sportovců
| Title in English | Content of lysozyme in saliva of athlets |
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| Authors | |
| Year of publication | 2022 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Objectives: The aim of the work was to determine changes in the concentration of lysozyme after a rest period and after sports. Material and methodology: 12 sports gymnasts - boys aged 8-18 years old - took part in the research. A saliva sample was collected from these athletes after a period of rest and then after a period of intense training. The collected saliva sample was subsequently centrifuged, the clear part was withdrawn with a pipette into a plastic tube. After that, the sample was given the number of the examined athlete and immediately frozen. Lysozyme was determined in saliva samples by simple radial diffusion in agarose. Lysozyme breaks down the polysaccharide murein, which is a basic component of gram-positive bacteria. It enzymatically disrupts the glycosidic bond between N-acetylglucosamine and N-acetylmuramic acid. This method uses Micrococcus luteus, which belongs to the most sensitive microorganisms dissolved by lysozyme. First, agarose plates were prepared. The agarose was mixed with borate-phosphate buffer to which Micrococcus luteus had been added. The mixture was boiled until the agarose completely dissolved and the Micrococcus luteus cells were completely dispersed. It was then poured onto the lids of the microtitre plates. After it cooled and solidified, wells were cut into the gel. A calibration series and saliva samples were pipetted into the wells. The plates were then incubated in a humid chamber. After incubation, the diameter of the diffusion zone was read using a special gauge. The amount of lysozyme in the sample was determined by reading from the calibration curve constructed according to the calibration series. Results and discussion: It was found that after physical exertion there was a decrease in the level of lysozyme in all monitored sports gymnasts. Similar results were reached by Vest with the team (2010) among adult rowers and Cunniffe with the team (2011) among rugby players. Conclusion: The changes in lysozyme concentration after the rest period and after the cessation of intensive training measured in this work show its potential as a possible biomarker of long-term sports load (Váňa et al., 2020). So far, it was a pilot study with a small number of participants, which will need to be verified on a larger group of athletes of both sexes. |
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