Asociace tkáňové exprese miR-196a s Barrettovým jícnem a adenokarcinomem jícnu
| Title in English | Association of tissue expression of miR-196a with Barrett's esophagus and esophageal adenocarcinoma |
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| Authors | |
| Year of publication | 2023 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Esophageal adenocarcinoma (EAC) is a highly aggressive form of cancer that often develops from Barrett's esophagus (BE), a premalignant pathological change in the lower esophagus. Specific microRNAs (miRNAs), small non-coding RNAs that function as post-transcriptional regulators of gene expression, have been repeatedly shown to play a key role in the pathogenesis of these diseases. This pilot study aimed to analyze four selected miRNAs in esophageal tissue of healthy controls (HC) and patients with reflux esophagitis (RE)/BE/EAC. Another aim was to compare expression in Barrett's esophagus/adenocarcinoma tissue and in tissue outside the main pathology in BE/EAC patients. Twenty-two subjects (3 HC, 8 RE, 5 BE, 6 EAC) who underwent endoscopic examination were included in the study. RNA was isolated from frozen esophageal tissue samples (preserved in RNAlater™ Stabilization Solution at -70°C) using the AllPrep DNA/RNA/miRNA Universal Kit. Subsequent RT-qPCR analysis was performed using selected TaqMan MicroRNA Assays for miR21, miR34a, miR196a, miR196b and endogenous controls (RNU44).? While miR-21 expression in pathological esophageal tissue was decreased in BE and EAC patients compared to HC and RE patient groups (p=0.01), miR-196a expression was increased in BE and EAC patients (p<0.01). A correlation was observed between the expression of these two miRNAs and the severity of diagnosis (p<0.05). In addition, we observed that miR-196a was significantly more expressed at the site with the main pathology compared to paired adjacent esophageal tissue in BE and EAC patients (p<0.01). In previous studies, miR-196a has been associated with esophageal squamous cell carcinoma and is therefore viewed as a potential therapeutic target in this disease. Consistent with the studies by Luzno et al. and Maru et al, the results of our pilot study suggest that miR-196a, which regulates cell proliferation, invasion and migration, could become a suitable diagnostic tissue biomarker for BE and EAC. |
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