Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

SMOLKOVÁ Denisa ČMELÍK Richard LAVICKÁ Jana

Year of publication 2023
Type Article in Periodical
Magazine / Source TrAC Trends in Analytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1016/j.trac.2023.117068
Doi http://dx.doi.org/10.1016/j.trac.2023.117068
Keywords Capillary electrophoresis; Derivatization; Fluorescence; Glycans; Glycomics; Labeling; Mass spectrometry; Oligosaccharides
Description Capillary electrophoresis represents a powerful separation technique that has been widely accepted in oligosaccharide and glycan analysis. However, due to their low electrophoretic mobility as well as the lack of chromophore or fluorophore in their native structure, appropriate modification of the saccharide molecule is required before separation and detection. In this review, we summarize the labeling strategies used for oligosaccharide/glycan analysis followed by electrophoretic separation published between 2010 and mid-2022. The review is divided by the labeling mechanism and part of the saccharide molecule used for modification. Although most labeling for CE-based applications is done with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, we have also summarized less common, rare, and very recently developed tags with applicability in glycomics.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.