Microbiome and pharmacotherapy in patients with gastrointestinal disorders

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Authors	MLČŮCHOVÁ Natálie BOŘILOVÁ LINHARTOVÁ Petra
Year of publication	2023
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	Background: Etiopathogenesis of gastrointestinal disorders (GDs), such as gastroesophageal reflux disease (GERD) and its complications, pancreatic cancer (PANC), and colorectal adenocarcinoma (CRC) is multifactorial; risk factors for disease development and/or progression include external factors, dysbiosis of host microbiota, genetic predisposition, and responsiveness to therapy. Objectives: The main aim of the dissertation project is to identify potential markers at the level of the host microbiome. Methods: Human and microbial DNA will be isolated from blood and biopsies from the digestive tract. Microbial DNA will be analyzed by 16S rRNA amplicon sequencing and quantified by multiplex qPCR method. Outcomes: Paired comparison of bacteriomes between healthy and pathological parts of the digestive tract will be performed to identify potentially risky host bacteriome profiles.
Related projects:	Host microbiome in relation to Barrett ´s esophagus and esophageal adenocarcinoma development