Animal model of mucosal inflammation (gastritis) and its application in balneology
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| Year of publication | 2023 |
| Type | Conference abstract |
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| Description | Inflammatory diseases of the mucous membrane located in various organs of our body, such as the gastrointestinal tract or respiratory system, are common diseases of children and adults. Since the end of the 17th century, the positive effects of natural mineral water Vincentka from Luhačovice Spa (Czech Republic) on inflamatory diseases have been empirically known. The aim of this pilot study is to verify on an animal model of gastritis - as an example of mucosal inflammation - empirically reported improvement in health during drinking therapy of Vincentka natural mineral water. Sixteen Wistar laboratory rats (one-month-old males, weight 240-280 g) used in the experiment were divided into two groups. Control group of animals (Co) received standard water at a daily dose of 0.9 ml/100 g of their current body weight through an orogastric tube for the first 7 days, and the other group of animals (Vi) received the same dose of Vincentka instead of water. Before starting the application of liquids, an adaptation phase (handling) took place in all animals for 1 week. On the eighth day, gastric inflammation was induced in both groups of animals using indomethacin solution at a dose 1 ml/100 g. Twenty-four hours after the administration of indomethacin, the experiment was terminated. Blood was collected by intracardiac puncture, accompanied by dissection and removal of the stomach and duodenum. The collected tissues and blood were further examined by an immunohistochemical and biochemical methods. The experiment was approved by Ministry of Education, Youth and Sports MSMT-35703/2019-2. We found significantly higher values of the parameters of the antioxidant capacity of the blood plasma in the Vi versus Co group: concentration of the sulfanyl group 225.5 (219.9-272.0) vs. 184.6 (108.4-234.8 µmol/L), p<0.05; 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid 0.104 (0.105-0.815) vs. 0.103 (0.102-0.104 (mgGA/ml)), p<0.05. Histological microscopy showed a reduced number of peptic erosions and ulcerations of the stomach in group Vi (p<0.01). We can assume that "Vincentka mineral water from a natural healing source" is probably the cause protecting the GIT mucosa from the effect of acute inflammation, increases the antioxidant capacity of the body and also reduces signs of oxidative stress. |
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