Determination of lamotrigine in alternative types of sample utilizing validated LC-MS method
| Authors | |
|---|---|
| Year of publication | 2023 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | This study presents the analytical method for antiepileptic drug analysis, focusing on the determination of lamotrigine (LTG) in saliva and dried blood spots (DBS). The significance of monitoring antiepileptic drugs, such as lamotrigine, in saliva and DBS lies in their potential applications for therapeutic drug monitoring and personalized medicine. Saliva, being a non-invasively collected and easily accessible biological fluid, offers an alternative to traditional blood sampling for determination of drug levels. DBS, as well, can offer reasonable option for the determination of drug concentrations with minimally invasive sampling from blood capillaries. The developed LC-MS method was subjected to an essential validation process. Liquid-liquid extraction was used for both types of biological matrix, employing ethyl acetate for LTG extraction from saliva samples and MeOH:ACN 3:1 (v/v) solution for extraction of DBS samples. The validated LC-MS method was successfully applied for LTG determination in patient saliva and DBS samples. The most of samples were obtained from patients on polypharmacotherapy (the study was approved by local Ethical committees, No. 32/2022 and 03V/2023). The presented method is suitable for the quantification of LTG in alternative matrices/DBS and saliva. |
| Related projects: |