Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
| Authors | |
|---|---|
| Year of publication | 2023 |
| Type | Article in Periodical |
| Magazine / Source | Microchimica Acta |
| MU Faculty or unit | |
| Citation | |
| Web | https://link.springer.com/article/10.1007/s00604-023-05873-1 |
| Doi | http://dx.doi.org/10.1007/s00604-023-05873-1 |
| Keywords | Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion |
| Description | An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers. |
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