ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH

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Authors

KUCHYNKA Michaela VLČNOVSKÁ Marcela KALLALA Kim BISKUPIČ Jan HLOŽKOVÁ Jana SCHEER Peter PADRTOVÁ Tereza OPATŘILOVÁ Radka VACULOVIČOVÁ Markéta KANICKÝ Viktor MASAŘÍK Michal VACULOVIČ Tomáš

Year of publication 2023
MU Faculty or unit

Faculty of Pharmacy

Citation
Description In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.
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