Microphysiological Analysis of Cardiomyocyte Function in 3D Organoids
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| Year of publication | 2024 |
| Type | Conference abstract |
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| Description | Human cardiomyocytes differentiated from pluripotent stem cell lines in 3D organoids allow non- invasive and long-term functional analysis of the organ-on-a-chip type. The functional phenotype can be described by a set of three methods: mechanical response mediated by the cantilever of the atomic force microscope (AFM), cellular electrophysiology by means of the microelectrode field (MEA), and calcium homeostasis by means of Ca imaging. This electrical/nanomechanical combination provides a real-time link of electrophysiology to the contracting force of cardiomyocytes. In various settings, the methods allow testing of new and known drugs, including combinations. For example, in pulmonary bronchodilators, it was thus possible to describe their relationship to cardiac arrhythmias. Testing is then possible in cells without known mutations as well as in lines of specific hereditary diseases (e.g., Duchenne muscular dystrophy, or catecholaminergic polymorphic ventricular tachycardia). The methodology, as well as clinical relevance, will be presented together with a new model of dual biosensor, for the first time allowing the study of conduction aspects of arrhythmia. The work was supported by AZV grant NU20-06-00156 and by the project National Institute for Research of Metabolic and Cardiovascular Diseases (Programme EXCELES, ID Project No. LX22NPO5104) – Funded by the European Union – Next Generation EU. |
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