Amyloid precursor protein induces reactive astrogliosis

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Authors

JAUREGUI Gretsen Velezmoro VUKIĆ Dragana ONYANGO Isaac G ARIAS Carlos NOVOTNY Jan S TEXLOVA Katerina WANG Shanshan KOVACOVICOVA Kristina Locker POLAKOVA Natalie ZELINKOVA Jana CARNA Maria LACOVICH STRAŠIL Valentina HEAD Brian P HAVAS Daniel MISTRIK Martin ZOREC Robert VERKHRATSKY Alexei KEEGAN Liam O'CONNELL Mary Anne RISSMAN Robert STOKIN Gorazd B

Year of publication 2024
Type Article in Periodical
Magazine / Source Acta Physiologica
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://onlinelibrary.wiley.com/doi/10.1111/apha.14142
Doi http://dx.doi.org/10.1111/apha.14142
Keywords amyloid precursor protein; astrocytes; interferon pathway; lipopolysaccharide; reactive astrogliosis; traumatic brain injury
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Description AimAstrocytes respond to stressors by acquiring a reactive state characterized by changes in their morphology and function. Molecules underlying reactive astrogliosis, however, remain largely unknown. Given that several studies observed increase in the Amyloid Precursor Protein (APP) in reactive astrocytes, we here test whether APP plays a role in reactive astrogliosis.MethodsWe investigated whether APP instigates reactive astroglios by examining in vitro and in vivo the morphology and function of naive and APP-deficient astrocytes in response to APP and well-established stressors.ResultsOverexpression of APP in cultured astrocytes led to remodeling of the intermediate filament network, enhancement of cytokine production, and activation of cellular programs centered around the interferon (IFN) pathway, all signs of reactive astrogliosis. Conversely, APP deletion abrogated remodeling of the intermediate filament network and blunted expression of IFN-stimulated gene products in response to lipopolysaccharide. Following traumatic brain injury (TBI), mouse reactive astrocytes also exhibited an association between APP and IFN, while APP deletion curbed the increase in glial fibrillary acidic protein observed canonically in astrocytes in response to TBI.ConclusionsThe APP thus represents a candidate molecular inducer and regulator of reactive astrogliosis. This finding has implications for understanding pathophysiology of neurodegenerative and other diseases of the nervous system characterized by reactive astrogliosis and opens potential new therapeutic avenues targeting APP and its pathways to modulate reactive astrogliosis.
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